With the development of human production activities, environmental pollution caused by industrial and agricultural wastewater has attracted widespread attention. Among them, heavy metal pollutants alter the function and structure of proteins and produce reactive oxygen species, which have harmful effects on human health. Heavy metals are harmful to most organisms, including humans, and organisms have developed many protective systems to reduce the toxicity of heavy metals. Our C. elegans heavy metal analysis service can help researchers gain insight into the pathomechanisms of human diseases associated with heavy metals and screen for relevant drugs.

Heavy Metal Stress Assay in C. elegans

Heavy metals such as lead, cadmium, mercury, and arsenic are widespread environmental pollutants, and their bioaccumulation can lead to serious human diseases such as cancer, neurodegenerative diseases, and renal dysfunction. Due to the complexity of mammalian systems, it is difficult to elucidate the mechanisms of heavy metal detoxification in mammals. C. elegans is an excellent model for gaining insight into the toxicity mechanisms, cellular responses and potential remediation strategies associated with heavy metal contamination due to its conserved biological pathways and ease of manipulation. Existing studies have shown that many genes in C. elegans, including detoxification enzymes, transcription factors and signaling factors, are involved in the protection against heavy metals.

Fig.1 Caenorhabditis elegans as a Model to Study Manganese-Induced Neurotoxicity. (Martins A C, et al., 2022)

Our Services

As an excellent model organism, C. elegans is widely used in many fields such as biochemistry, neuroscience, pharmacology, and toxicology, etc. CD BioSciences provides professional C. elegans heavy metal analysis services by monitoring reporter genes and quantifying the growth and survival of C. elegans after exposure to heavy metals. Our services are listed below:

Establishment of the Heavy Metal Model

• Customize your solution based on the heavy metals you are interested in.
• Options: Cadmium (Cd), copper (Cu), lead (Pb) and zinc (Zn), etc.

Assessment of Heavy Metal Toxicity

• Two physiological endpoints: growth and reproduction.
• Three behavioral endpoints: head thrash frequency, body bend frequency and feeding.
• Two enzymatic endpoints: acetylcholine esterase (AChE) and superoxide dismutase (SOD).

Monitoring Response to Heavy Metal

• Acute reporter assay in liquid.
• Acute reporter assay on dish.

Why Choose Us?

CD BioSciences is committed to providing the best services to accelerate our clients' research goals. Our highly trained scientists provide you with customized solutions on C. elegans research with our cutting-edge technology and expertise. We are confident that we can help you gain insights into the pathology of human diseases associated with heavy metals and provide a suitable platform for the development of screening systems for related drugs. If you are interested in our services or have any steady state, please feel free to contact us.

Reference

1. Martins A C, Gubert P, Li J, et al. Caenorhabditis elegans as a model to study manganese-induced neurotoxicity[J]. Biomolecules, 2022, 12(10): 1396.

Related Services

• C. elegans Oxidative Stress Analysis Service
• C. elegans Heat Shock Stress Analysis Service
• C. elegans Osmotic Stress Analysis Service
• C. elegans Cold Tolerance Analysis Service
• C. elegans Hypoxia Analysis Service
• C. elegans UV Stress Analysis Service