For the model system organism C. elegans, microinjection is a common technique used for DNA transformation. This technique is powerful to assemble genetic and molecular analysis to phenotypic analysis, facilitating the transgenic expression of genes, and the implementation of technologies, such as clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system and RNA interference (RNAi). This technology brings important insights to genetics, neurobiology, developmental biology, and disease research. At CD BioSciences, we are committed to providing you with accurate and efficient C. elegans custom injections to meet your specific needs.

Introduction to C. elegans Microinjection

Microinjection is a fundamental technique used in C. elegans genetic and developmental studies that allows researchers to introduce specific molecules, such as DNA, RNA, proteins, or other compounds into C. elegans embryos or larval cells to study gene function, cellular processes, and developmental pathways with great precision. The microinjection procedure is administered through the reproductive system of C. elegans. The correct site performed microinjection for genetic transformation in C. elegans is the syncytial gonad, which ensures the incorporation of injected DNA into the nuclei of oocytes, and finally inherited through the fertilized egg.

Fig.1 Microinjection scheme for C. elegans hermaphrodite.

Our Services

As a leading global provider of customized services, CD BioSciences is proud to offer cutting-edge C. elegans microinjection services. With an advanced and proven technology platform, experienced scientists skillfully maneuver fine glass needles or micropipettes through different samples, including plasmids, DNA, PCR products, and other substances of interest to you. We confirmed the survival of injected animals 24 hours after injection.

Workflow of Our Services

Applications for C. elegans Microinjection

• For the phenotypic rescue of mutants
• For the analysis of gene expression (combining with reporter gene assays) and tagged proteins
• For the analysis of structure and function of protein domains
• Enable to facilitate the implementation of technologies such as gene editing and RNAi.
• Enable to inject a number of other chemicals of interest, such as drugs, proteins and small RNA molecules into the gonad or other distinct tissues.

Why Choose CD BioSciences?

CD BioSciences is a professional C. elegans model service provider. Microinjection is an essential tool for C. elegans research, involving creating transgenic worms, for RNAi of selected genes, and readily adapted for introducing various types of molecules directly to various locations. With our advanced equipment and scientists with years of experience, we are confident to offer our customers with high-quality data delivery, in-depth scientific and technical support. Please don't hesitate to contact us for more details.

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