Oxygen consumption rate (OCR) is an important marker of cellular function under normal cellular or metabolically challenging conditions and is widely used in studies of mitochondrial function or of factors mediating the switch from oxidative phosphorylation to aerobic glycolysis. Cellular oxygen consumption is a fundamental indicator of mitochondrial function, reflecting cellular reactive oxygen ROS production and metabolic activity. Measurement of C. elegans oxygen consumption is a valid measure of toxicity, especially in the context of metal contamination. For C. elegans oxygen consumption rate measurements, CD BioSciences has an advanced technology platform to help you advance your project research.

C. elegans Oxygen Consumption Rate Measurement for Toxicity Testing

Mitochondria are critical to the health of the body because of their involvement in a variety of physiological activities, including energy production, reactive oxygen species (ROS) scavenging, calcium homeostasis and apoptosis. For cells with high energy demands, it is extremely important to have optimal mitochondrial function. Mitochondrial dysfunction is associated with many aging and neurodegenerative diseases. Measurement of oxygen consumption (OCR) in vivo or in vitro is valuable for biological systems.

Fig.1 Schematic overview of C. elegans OCR measurement using the Seahorse XFe96 Extracellular Flux Analyzer. (Preez GD, et al., 2020)

The functional or biologically relevant phenotypes exhibited by C. elegans as a model organism for toxicity testing and its use in high-throughput assessment protocols are often used as bioindicators for toxicity measurements. Phenotypes such as feeding, growth, locomotion, and reproduction of C. elegans are commonly used as toxicity endpoints, but its respiration has also been used to study the effects of toxicant exposure. This endpoint is a useful measure of the toxicity of particularly metal contamination to microorganisms, daphnia, and earthworms, among others.

Our Services

With the development and advancement of new technologies, the relevance and applicability of OCR measurements have been greatly improved. CD BioSciences has advanced high throughput respirometers dedicated to providing you with high quality C. elegans oxygen consumption rate measurement services, facilitating the development of acute response protocols that measure C. elegans OCR.

Workflow of Our Services

Stock preparation and density bioassay of C. elegans food.	Growth and synchronization of C. elegans.	Preparation of toxicant solution and hydration of probes.	Preparation of C. elegans plate and run assays.	Data analysis, including food density bioassay analysis, etc.

CD BioSciences is a professional C. elegans model service provider. C. elegans is a simple but powerful model organism, which can be used to elucidate the many intricacies of mitochondrial function. Mitochondrial respiration is an insightful indicator of mitochondrial function and here we provide the state-of-the-art platform to measure respiration in C. elegans using developed respirometers. If you are interested in our services, please don't hesitate to contact us.

Reference

1. Preez G D, Fourie H, Daneel M, et al. Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XFe96 Extracellular Flux Analyzer[J]. Scientific Reports, 2020, 10(1): 4239.

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