Targeted changes in the genome offer the ability to address fundamentally important questions. Genome editing tools based on the CRISPR-Cas9 system are a breakthrough technology, which can precisely modify a genome in a relatively simple manner. As a research expert in C. elegans, CD BioSciences offers CRISPR/Cas9-based gene editing services.

How CRISPR/Cas9 Works

The system is RNA-based bacterial defense mechanisms designed to recognize and eliminate foreign DNA from invading bacteriophage and plasmids. They consist of a Cas endonuclease that is directed to cleave a target sequence by a guide RNA (gRNA). Therefore, the system can be co-opted to cleave any target sequence of choice by modifying the sequence of the gRNA, which is encoded by a "CRISPR/array" in the bacterial genome.

Fig.1 Schematic representation of the CRISPR-Cas9 genome editing approach in C. elegans. (Kim HM, et al., 2016)

Our Services

• C. elegans Gene Knockin by CRISPR/Cas9
  DNA fragments can be inserted into the C. elegans genome with precision and efficiency using our cutting-edge CRISPR/Cas9 knockout technology. CD BioSciences' knockin services include tagging services and point mutation services.
• C. elegans Gene Knockout by CRISPR/Cas9
  CRISPR/Cas9 is a common tool for biologists to understand gene function, and CD BioSciences offers you CRISPR/Cas9 knockout services that can help you study specific genes, proteins of interest, to determine their function.

Workflow of Our Services

CRISPR/Cas9 system was first adapted for C. elegans in 2013 and has been successfully applied to C. elegans nowadays. We have established an efficient genome editing platform for delivering genetically engineered C. elegans optimized to meet your specifications.

Customized CRISPR/Cas9 gene editing strategy	Design and synthesize gRNA	CRISPR vector construction	Plasmid microinjection	Screening and verification	Deliver the C. elegans model and report

• High-efficiency CRISPR technology platform.
• Whole-process technical trace service.
• Convenient and efficient transportation service.
• One-stop solutions.
• Most competitive prices.

CD BioSciences offers a series of CRISPR/Cas9 C. elegans model customization services that are part of our integrated portfolio of custom C. elegans model development services. We are committed to offering convenient and efficient transportation services with shorter turnaround time and lower prices. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us for more details.

Reference

1. Kim H M, Colaiácovo M P. CRISPR‐Cas9‐guided genome engineering in C. elegans[J]. Current Protocols in Molecular Biology, 2016, 115(1): 31.7. 1-31.7. 18.

Related Services

• C. elegans MosDel Service
• C. elegans MosSCI Service
• C. elegans Array and Integration Service
• C. elegans Gene Knockin by CRISPR/Cas9
• C. elegans Gene Knockout by CRISPR/Cas9