C. elegans Reporter Strain Construction Service
As a model organism, there is a high degree of homology between C. elegans and human pathways and is widely used in biological research. C. elegans reporter strains can be successfully constructed through array and integration and are widely used in studies of gene expression patterns, cellular localization, and promoter activity. CD BioSciences, an expert in C. elegans genetics and gene editing technologies, is confident to provide you with a wide range of C. elegans reporter strains expressing reporter genes (such as the GFP) with our advanced array and integration technology platform.
C. elegans Reporter Strain Construction by Array and Integration
Extrachromosomal arrays are useful for transient or temporary expression of transgenes in C. elegans. They provide a convenient and efficient way to study gene expression, regulatory elements, and other genetic manipulations in a controlled and heritable manner without directly integrating into the C. elegans's chromosomes. Extrachromosomal arrays are self-replicating and can persist for multiple generations in the C. elegans's germ line. Using array and integrated-based gene editing techniques, reporter genes can be stably integrated into the C. elegans genome by microinjection of foreign DNA constructs to construct C. elegans reporter strains.
Fig.1 Robust, tissue-specific labeling of a target protein at its native expression level. (He S, et al., 2019)
Application of C. elegans Reporter Strains in Biological Research
C. elegans reporter strains provide a valuable tool for studying gene regulation, cellular processes, and developmental biology, accelerating researchers' understanding of fundamental biological mechanisms.
- Genetic Screening: C. elegans reporter strains can help researchers visualize and study gene expression patterns, cellular processes and signaling events in living organisms, providing valuable insights into the function of genes and their role in development, behavior, and disease.
- Drug Screening: C. elegans reporter strains are widely used in drug screening experiments to assess the effects of compounds on specific cellular processes, gene expression patterns or neuronal function in C. elegans. It helps researchers to gain a comprehensive understanding of drug action and provides valuable insights into the therapeutic potential or mechanism of action of a drug.
- Gene Expression Analysis: C. elegans reporter strains fusing a reporter group (e.g., GFP) to a target gene enables visualization and quantitative studies of gene expression patterns in different tissues, developmental stages or under specific conditions.
- Promoter Characterization: By fusing promoter regions of genes of different lengths to reporter genes, researchers can identify and analyze specific promoter regions, enabling the study of promoter activity and regulatory elements.
Fig.2 Labeling Spatially Defined Protein Subpopulations. (Yuet KP, et al., 2015)
- Cell Lineage Tracing: Using reporter genes to tag target cells to track specific C. elegans cell lineages, which in turn helps researchers study cell division, migration, and differentiation during development.
- Subcellular Localization Studies: C. elegans reporter lines help researchers achieve subcellular localization studies of proteins in specific organelles, subcellular compartments, or specific cell types.
- Regulatory Element Functional Analysis: C. elegans reporter strains can be used to study the function and regulation of specific DNA regulatory elements, such as enhancers or silencers.
- Genetic Interaction Studies: By crossing reporter strains with mutant strains, researchers can assess gene-to-gene interactions.
Our Services
As experts in C. elegans genetics and gene editing technologies, CD BioSciences has developed an advanced and comprehensive technology platform for the construction of the reporting strain of C. elegans, using arrays and integration to achieve the reporting gene expression.
Workflow of Our Services
Design the Reporter Construct
- Clone the promoter region upstream of the reporter gene (e.g., GFP) into a plasmid vector along with any other desired elements.
Generation of Extrachromosomal Arrays
- Prepare the reporter construct
- Microinjection
- Selection
- Screen for desired expression elements.
Integration via Homologous Recombination
- Design the targeting construct
- Microinjection
- Selection and screening
- Confirmation of integration
Characterization And Maintenance
- Maintain the reporter strain by propagating it through successive generations to ensure stable inheritance of the integrated reporter construct.
Features of Our Services
- Advanced technology platform
- Excellent team of experts
- Whole-process technical trace service
- Shorter turnaround time and lower prices
At CD BioSciences, our experienced scientists are experienced in C. elegans reporter strains. After communicating with you about your test protocol, we will implement your protocol within a quick turnaround time and ensure the accuracy of your results, so please feel free to contact us if you have any questions or specific needs.
References
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He S, Cuentas-Condori A, Miller III D M. NATF (Native and Tissue-Specific Fluorescence): a strategy for bright, tissue-specific GFP labeling of native proteins in Caenorhabditis elegans[J]. Genetics, 2019, 212(2): 387-395.
- Yuet K P, Doma M K, Ngo J T, et al. Cell-specific proteomic analysis in Caenorhabditis elegans[J]. Proceedings of the National Academy of Sciences, 2015, 112(9): 2705-2710.
For research use only.
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