The phenomenon of RNA interference (RNAi) was first identified in C. elegans, where gene specific sequences are knocked out by the introduction of double-stranded RNA (dsRNA) and is suitable for large-scale screens to interrogate the function of various model organisms and other species. RNAi screening is often performed in cultured or primary cell species, or in model organisms such as C. elegans. Through many RNAi screens can facilitate new insights into different organisms and biomedicine for researchers. CD BioSciences has extensive experience and a high throughput technology platform for RNAi screening in C. elegans. Combining experience and technology, we are confident that we can provide a high quality, cost effective and bespoke service to our customers worldwide.

Introduction to RNAi Screening

RNAi is a powerful tool for testing gene function, and the results of RNAi screening help researchers gain a new understanding of gene function and network in the context of basic cell biology and biomedicine. As a powerful reverse genetic tool, RNAi has revolutionized genetic research in model organisms. Targeted gene knockout using high-throughput screening is becoming a common approach in science, allowing researchers to assess the function of every gene in a genome in a relatively short period of time.

• Like genetic screening, RNAi screening allows the identification of genes associated with specific pathways, structures, or functions by correlating mutant phenotypes with gene knockouts.
• Like chemical screening, RNAi screening is suitable for miniaturization and automation, which is conducive to high-throughput research.
• C. elegans, Drosophila cells and mammalian cells have been the most commonly used RNAi screening systems. Large-scale RNAi screens have led researchers to new insights into gene function associated with topics such as infection, cancer, obesity, and aging.
• In addition, RNAi screening benefits from rapid developments in various other areas, such as improved automated acquisition and analysis methods for high-content images.

Fig.1 Schematic of C. elegans RNAi screening assay protocol. (Jagadeesan S, et al., 2018)

RNAi Screening in C. elegans

As with other large-scale data acquisitions, such as the integration of DNA microarrays and protein-protein interaction maps, high-throughput RNAi screening has increased the speed and reliability of screening and accelerated our understanding of organisms at the genetic level. C. elegans is a model tool for ageing, neurobiology, cell migration, germline-specific processes, and disease.

Whereas classical genetics (forward genetics) has traditionally been the primary means of studying C. elegans gene function, RNAi as a reverse genetics' technique leverages our knowledge of the near-complete gene complement of an organism, allowing for the simultaneous study of germline-specific function in all cell types. In C. elegans, RNAi is systematic and heritable. Unlike mammalian systems, when dsRNAs are introduced into C. elegans, they do not induce the interferon response that is prevalent in mammalian cells.

Our Services

As a leading global provider of custom services, CD BioSciences has been at the forefront of C. elegans screening services with our extensive experience and high-throughput technology platform. We offer high-quality, customized, cost-effective RNAi screening services to take your research to the next level.

• The advanced technology platform and an excellent team of experts.
• High-quality services and products at competitive prices.
• Timely report on project progress.
• Our clients can interact with our scientists directly for prompt responses.

For many years, CD BioSciences has focused on providing C. elegans screening services to passionate researchers worldwide. With our sophisticated equipment and extensive experience, our scientists are confident that we can meet each of your specific needs. If you are interested in our services or have any additional questions, please feel free to contact us.

Reference

1. Jagadeesan S, Hakkim A. RNAi Screening: Automated High‐Throughput Liquid RNAi Screening in Caenorhabditis elegans[J]. Current protocols in molecular biology, 2018, 124(1): e65.

Related Services

• C. elegans Targeted RNAi Service